Sec-Delivered Effector 1 (SDE1) of 'Candidatus Liberibacter asiaticus' Promotes Citrus Huanglongbing.
Identifieur interne : 000055 ( Main/Exploration ); précédent : 000054; suivant : 000056Sec-Delivered Effector 1 (SDE1) of 'Candidatus Liberibacter asiaticus' Promotes Citrus Huanglongbing.
Auteurs : Kelley J. Clark [États-Unis] ; Zhiqian Pang [États-Unis] ; Jessica Trinh [États-Unis] ; Nian Wang [États-Unis] ; Wenbo Ma [États-Unis]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2020.
Abstract
Sec-delivered effector 1 (SDE1) from the huanglongbing (HLB)-associated bacterium 'Candidatus Liberibacter asiaticus' was previously characterized as an inhibitor of defense-related, papain-like cysteine proteases in vitro and in planta. Here, we investigated the contributions of SDE1 to HLB progression. We found that SDE1 expression in the model plant Arabidopsis thaliana caused severe yellowing in mature leaves, reminiscent of both 'Ca. L. asiaticus' infection symptoms and accelerated leaf senescence. Induction of senescence signatures was also observed in the SDE1-expressing A. thaliana lines. These signatures were apparent in older leaves but not in seedlings, suggesting an age-associated effect. Furthermore, independent lines of transgenic Citrus paradisi (L.) Macfadyen (Duncan grapefruit) that express SDE1 exhibited hypersusceptibility to 'Ca. L. asiaticus'. Similar to A. thaliana, transgenic citrus expressing SDE1 showed altered expression of senescence-associated genes, but only after infection with 'Ca. L. asiaticus'. These findings suggest that SDE1 is a virulence factor that contributes to HLB progression, likely by inducing premature or accelerated senescence in citrus. This work provides new insight into HLB pathogenesis.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
DOI: 10.1094/MPMI-05-20-0123-R
PubMed: 32986514
Affiliations:
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Clark</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Pang, Zhiqian" sort="Pang, Zhiqian" uniqKey="Pang Z" first="Zhiqian" last="Pang">Zhiqian Pang</name><affiliation wicri:level="2"><nlm:affiliation>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Trinh, Jessica" sort="Trinh, Jessica" uniqKey="Trinh J" first="Jessica" last="Trinh">Jessica Trinh</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name><affiliation wicri:level="2"><nlm:affiliation>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Ma, Wenbo" sort="Ma, Wenbo" uniqKey="Ma W" first="Wenbo" last="Ma">Wenbo Ma</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sec-delivered effector 1 (SDE1) from the huanglongbing (HLB)-associated bacterium '<i>Candidatus</i> Liberibacter asiaticus' was previously characterized as an inhibitor of defense-related, papain-like cysteine proteases in vitro and in planta. Here, we investigated the contributions of SDE1 to HLB progression. We found that SDE1 expression in the model plant <i>Arabidopsis thaliana</i> caused severe yellowing in mature leaves, reminiscent of both '<i>Ca.</i> L. asiaticus' infection symptoms and accelerated leaf senescence. Induction of senescence signatures was also observed in the SDE1-expressing <i>A. thaliana</i> lines. These signatures were apparent in older leaves but not in seedlings, suggesting an age-associated effect. Furthermore, independent lines of transgenic <i>Citrus paradisi</i> (L.) Macfadyen (Duncan grapefruit) that express <i>SDE1</i> exhibited hypersusceptibility to '<i>Ca.</i> L. asiaticus'. Similar to <i>A. thaliana</i>, transgenic citrus expressing SDE1 showed altered expression of senescence-associated genes, but only after infection with '<i>Ca.</i> L. asiaticus'. These findings suggest that SDE1 is a virulence factor that contributes to HLB progression, likely by inducing premature or accelerated senescence in citrus. This work provides new insight into HLB pathogenesis.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32986514</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Nov</Month><Day>03</Day></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Sec-Delivered Effector 1 (SDE1) of '<i>Candidatus</i> Liberibacter asiaticus' Promotes Citrus Huanglongbing.</ArticleTitle><Pagination><MedlinePgn>MPMI05200123R</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-05-20-0123-R</ELocationID><Abstract><AbstractText>Sec-delivered effector 1 (SDE1) from the huanglongbing (HLB)-associated bacterium '<i>Candidatus</i> Liberibacter asiaticus' was previously characterized as an inhibitor of defense-related, papain-like cysteine proteases in vitro and in planta. Here, we investigated the contributions of SDE1 to HLB progression. We found that SDE1 expression in the model plant <i>Arabidopsis thaliana</i> caused severe yellowing in mature leaves, reminiscent of both '<i>Ca.</i> L. asiaticus' infection symptoms and accelerated leaf senescence. Induction of senescence signatures was also observed in the SDE1-expressing <i>A. thaliana</i> lines. These signatures were apparent in older leaves but not in seedlings, suggesting an age-associated effect. Furthermore, independent lines of transgenic <i>Citrus paradisi</i> (L.) Macfadyen (Duncan grapefruit) that express <i>SDE1</i> exhibited hypersusceptibility to '<i>Ca.</i> L. asiaticus'. Similar to <i>A. thaliana</i>, transgenic citrus expressing SDE1 showed altered expression of senescence-associated genes, but only after infection with '<i>Ca.</i> L. asiaticus'. These findings suggest that SDE1 is a virulence factor that contributes to HLB progression, likely by inducing premature or accelerated senescence in citrus. This work provides new insight into HLB pathogenesis.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Clark</LastName><ForeName>Kelley J</ForeName><Initials>KJ</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pang</LastName><ForeName>Zhiqian</ForeName><Initials>Z</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1854-1414</Identifier><AffiliationInfo><Affiliation>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Trinh</LastName><ForeName>Jessica</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Nian</ForeName><Initials>N</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-7743-0728</Identifier><AffiliationInfo><Affiliation>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Wenbo</ForeName><Initials>W</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-5569-639X</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">HLB</Keyword><Keyword MajorTopicYN="N">bacterial pathogenesis</Keyword><Keyword MajorTopicYN="N">citrus greening</Keyword><Keyword MajorTopicYN="N">pathogen effectors</Keyword><Keyword MajorTopicYN="N">senescence</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>28</Day><Hour>17</Hour><Minute>10</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32986514</ArticleId><ArticleId IdType="doi">10.1094/MPMI-05-20-0123-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Clark, Kelley J" sort="Clark, Kelley J" uniqKey="Clark K" first="Kelley J" last="Clark">Kelley J. Clark</name></region><name sortKey="Ma, Wenbo" sort="Ma, Wenbo" uniqKey="Ma W" first="Wenbo" last="Ma">Wenbo Ma</name><name sortKey="Pang, Zhiqian" sort="Pang, Zhiqian" uniqKey="Pang Z" first="Zhiqian" last="Pang">Zhiqian Pang</name><name sortKey="Trinh, Jessica" sort="Trinh, Jessica" uniqKey="Trinh J" first="Jessica" last="Trinh">Jessica Trinh</name><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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